A defined medium for a fastidious Spiroplasma.

  title={A defined medium for a fastidious Spiroplasma.},
  author={Kevin Hackett and Allen S. Ginsberg and Shlomo Rottem and Roberta Henegar and Robert F. Whitcomb},
  volume={237 4814},
A defined medium (H-1) was developed for cultivation of the suckling mouse cataract agent, Spiroplasma mirum, a fastidious member of the class Mollicutes that causes cataracts and chronic brain infection in inoculated neonate mice. The H-1 medium was used to show the importance of sphingomyelin as a growth factor for the culture of the spiroplasma in vitro. The growth of Spiroplasma mirum and the pathology it induces in sphingomyelin-rich tissues in vivo may be related to this dependency.