A defined growth medium for Clostridium difficile.

@article{Karasawa1995ADG,
  title={A defined growth medium for Clostridium difficile.},
  author={Tadahiro Karasawa and Sinya Ikoma and Koji Yamakawa and Shin-ichi Nakamura},
  journal={Microbiology},
  year={1995},
  volume={141 ( Pt 2)},
  pages={371-5}
}
Minimal requirements of amino acids and vitamins were determined in chemically defined medium for five strains of Clostridium difficile. Cysteine, isoleucine, leucine, proline, tryptophan and valine were essential amino acids for growth of C. difficile. Arginine, glycine, histidine, methionine and threonine enhanced growth. Biotin, pantothenate and pyridoxine were essential vitamins. A defined medium containing the minimal requirements of amino acids and vitamins produced a rapid and heavy… CONTINUE READING
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