A deep profiler's guide to cytometry.

  title={A deep profiler's guide to cytometry.},
  author={Sean C Bendall and Garry P. Nolan and Mario Roederer and Pratip K. Chattopadhyay},
  journal={Trends in immunology},
  volume={33 7},
In recent years, advances in technology have provided us with tools to quantify the expression of multiple genes in individual cells. The ability to measure simultaneously multiple genes in the same cell is necessary to resolve the great diversity of cell subsets, as well as to define their function in the host. Fluorescence-based flow cytometry is the benchmark for this; with it, we can quantify 18 proteins per cell, at >10 000 cells/s. Mass cytometry is a new technology that promises to… CONTINUE READING
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