A damaged genome’s transcriptional landscape through multilayered expression profiling around in situ-mapped DNA double-strand breaks

  title={A damaged genome’s transcriptional landscape through multilayered expression profiling around in situ-mapped DNA double-strand breaks},
  author={Fabio Iannelli and Alessandro Galbiati and Ilaria Capozzo and Quan Nguyen and Brian Magnuson and Flavia Michelini and Giuseppina D'Alessandro and Matteo Cabrini and Marco Roncador and Sofia Francia and Nicola Crosetto and Mats Ljungman and Piero Carninci and Fabrizio d’Adda di Fagagna},
  journal={Nature Communications},
Of the many types of DNA damage, DNA double-strand breaks (DSBs) are probably the most deleterious. Mounting evidence points to an intricate relationship between DSBs and transcription. A cell system in which the impact on transcription can be investigated at precisely mapped genomic DSBs is essential to study this relationship. Here in a human cell line, we map genome-wide and at high resolution the DSBs induced by a restriction enzyme, and we characterize their impact on gene expression by… 

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