A cytochrome c fusion protein domain for convenient detection, quantification, and enhanced production of membrane proteins in Escherichia coli—Expression and characterization of cytochrome‐tagged Complex I subunits

@article{Gustavsson2010ACC,
  title={A cytochrome c fusion protein domain for convenient detection, quantification, and enhanced production of membrane proteins in Escherichia coli—Expression and characterization of cytochrome‐tagged Complex I subunits},
  author={T. Gustavsson and Maria Trane and V. Moparthi and Egle Miklovyte and Lavanya Moparthi and K. G{\'o}recki and Thom Leiding and S. P. {\AA}rsk{\"o}ld and C. H{\"a}gerh{\"a}ll},
  journal={Protein Science},
  year={2010},
  volume={19}
}
Overproduction of membrane proteins can be a cumbersome task, particularly if high yields are desirable. NADH:quinone oxidoreductase (Complex I) contains several very large membrane‐spanning protein subunits that hitherto have been impossible to express individually in any appreciable amounts in Escherichia coli. The polypeptides contain no prosthetic groups and are poorly antigenic, making optimization of protein production a challenging task. In this work, the C‐terminal ends of the Complex I… Expand
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