A cytochemical study of the leaf-gland enzymes of insectivorous plants of the genus Pinguicula

  title={A cytochemical study of the leaf-gland enzymes of insectivorous plants of the genus Pinguicula},
  author={Yolande Heslop-Harrison and Robert Bruce Knox},
SummaryCytochemical methods have been used to study the distribution of acid phosphatase, esterase, ribonuclease, amylase and protease activity in the stimulated and unstimulated leaf glands of Pinguicula grandiflora, P. vulgaris, P. lusitanica, and P. caudata. Two gland types are present, stalked and sessile. The stalked glands bear a muco-polysaccharide secretion droplet, and are concerned with capture of the prey; the sessile glands are specialised for digestion. In unstimulated glands of… 

Fluorescence labelling of phosphatase activity in digestive glands of carnivorous plants.

It is suggested that the inflorescence of Byblis and some Pinguicula species might also be an additional "carnivorous organ", which can trap a prey, digest it, and finally absorb available nutrients.

Defense and carnivory: Dual role of bracts inPassiflora foetida

Results suggest a novel role for bracts where primary function is to minimize predatory damage to developing flowers and fruits and the mechanism to digest the trapped insects to obtain free aminoacids.

Chemonastic Stalked Glands in the Carnivorous Rainbow Plant Byblis gigantea LINDL. (Byblidaceae, Lamiales)

Carnivorous rainbow plants (Byblis, Byblidaceae, Lamiales) possess sticky flypaper traps for the capture, retention, and digestion of prey (mainly small insects). The trapping system is based on a

Glands of carnivorous plants as a model system in cell biological research

In Drosera capensis, Byblis liniflora and Nepenthes *coccinea, the Golgi apparatus and its vesicles during formation and secretion of trapping mucilage, the endoplasmic reticulum producing digestive enzymes, the movement of organelles along the cytoskeleton are analyzed.

Evidence of protocarnivory in triggerplants (Stylidium spp.; Stylidiaceae).

Australian triggerplants (Stylidium spp.; Stylidiaceae) trap small insects using mucilage-secreting glandular hairs held at various points on their inflorescence stems and flower parts, providing strong evidence of protocarnivory in Stylidium.

The digestive systems of carnivorous plants

A systematic perspective on the digestive and absorptive capacity of convergently evolved carnivorous plants is provided, with an emphasis on the forms and functions of glands.

Localization of beta-glycerophosphatase and Mg++-activated adenosine triphosphatase in a moss haustorium, and the relation of these enzymes to the cell wall labyrinth

These findings stress the role of the epidermis as an absorptive epithelium, and show clearly that the enzyme activity associated with the plasma surface is in no reciprocal ratio to the degree of amplification of the surface area.

Ultrastructural ontogeny of leaf cavity trichomes inAzolla implies a functional role in metabolite exchange

The timing of transfer cell differentiation, considered together with information from other studies, suggests that branched hairs may be involved in exchange of fixed nitrogen between the symbionts, while simple hairs may participate in Exchange of fixed carbon from Azolla to Anabaena.

Immunogold localization of callose and other cell wall components in pea nodule transfer cells

Investigation of labyrinthine wall intrusions revealed that wall ingrowths had an internal structure with small domains of callose suggesting the presence of channels or vents, and a lectinlike glycoprotein, PsNLEC-1, was localized in intercellular spaces associated with nodule transfer cells.

In vitro propagation of the alkaloid-producing rare African liana, Triphyophyllum peltatum (Dioncophyllaceae)

Triphyophyllum peltatum (Dioncophyllaceae), a West African liana containing pharmaceutically promising naphthylisoquinoline alkaloids, has successfully been raised in vitro and the unique carnivorous leaves that developed were only formed by regenerating plants on the supplemented medium and not on deficiency media.



Pollen-wall proteins: localization and enzymic activity.

The intine enzymes are very readily leachable, and their function is probably connected with the early nutrition of the pollen tube and the penetration of the stigma, and they may therefore be important in pollen allergenicity.


The present communication is concerned with a specialized type of transfer cell found in certain higher plants, and it is wished to present and interpret cytological information relevant to its translocatory function.

Cytochemical Localization of Enzymes in the Wall of the Pollen Grain

The storage of various hydrolytic enzymes in the pollen walls of ten species of flowering plants is reported, and it is shown that the site of deposition is the cellulosic intine in walls of several different structural types.


There is general agreement that insectivorous plants, mostly living under conditions where inorganic nutrients are scarce, may utilize substances from their prey, and it appears that substances other than those which might replace the products of photosynthesis are likely to be acquired with advantage by insectivory.

Blütenbildung von Pinguicula lusitanica in vitro durch Fütterung mit Pollen

The most spectacular effect of the pollen feeding was an initiation of flowering, and during the following 6 months, the treated plants developed 127 flowers, the largest number on a single specimen being 14.

Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.

  • B. RotmanB. Papermaster
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1966
Results showed that AAC is an asparagine codon and that if the biological assembly of the polypeptide chains of proteins proceeds, as currently believed, from the NH2-terminal through the COOH-Terminal amino acid, the genetic code is translated by reading the messenger from the 5'- to the 3'-end of the polynucleotide chain.

[Development and flowering of Pinguicula lusitanica in axenic culture].

Pinguicula lusitanica in axenic agar culture showed significantly increased leaf development, more chlorophyll content and a greater number of flowers in response to feeding with Drosophila, egg yolk and ammonium phosphate.

Insectivorous Plants

IF further confirmation be needed of Mr. Darwin's discovery of absorption by the leaves of the Drosera rotundifolia it is afforded amply by the following experiments which I have just concluded

Excretion by glandular organs

Glands found on plants vary in size and complexity from simple unicellular glandular epidermal hairs or simple glandular surfaces to the complex multicellular tentacles found on carnivorous plants


‘[he use of freslsbv diazotizecl pararosanilin w-itim a-msaphthyb pisosphate its a sinmultaneous coupling azo dye method for acid phosphatase resulted in significant improveumment because of the nmaumy desirable characteristics of the final azo dyed (1, 2).