A cytochemical bioassay for arginine vasopressin: preliminary studies.

@article{Baylis1980ACB,
  title={A cytochemical bioassay for arginine vasopressin: preliminary studies.},
  author={Peter H Baylis and J Pitchfork and J. Chayen and Lucille Bitensky},
  journal={Journal of immunoassay},
  year={1980},
  volume={1 4},
  pages={399-411}
}
A new method of measuring vasopressin activity is described. It depends on the finding that the Na+-K+-ATPase activity, measured cytochemically, in the thick ascending limb of the loop of Henle in rat renal tissue maintained in vitro, responded to increasing concentrations of synthetic arginine vasopressin in a log-dose related fashion. The limit of sensitivity was 0.002 pg/ml (2 x 10(-15) mol/l). The dose-responses were reproducible; the inter-assay coefficient of variation was 6.4% at a… CONTINUE READING