A cryopreservation protocol for embryos of the endangered species Zizania texana.

@article{Walters2002ACP,
  title={A cryopreservation protocol for embryos of the endangered species Zizania texana.},
  author={Christina Walters and Darren H. Touchell and Paula Power and James Wesley-Smith and Michael F. Antolin},
  journal={Cryo letters},
  year={2002},
  volume={23 5},
  pages={291-8}
}
Seeds of the endangered species Zizania texana are recalcitrant, making it difficult to preserve the remaining genetic diversity of this species in genebanks. Excised embryos can be cryopreserved using solution-based cryoprotection protocols. Survival following cryoexposure increased from less than 5% to about 75% by preculturing embryos in high concentrations of sugars, bathing them in cryoprotectant solutions, and partially drying them to water contents of about 0.6 g H2O/g dry mass. 
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