Corpus ID: 12561288

A critical review of PCR primer design algorithms and cross-hybridization case study

  title={A critical review of PCR primer design algorithms and cross-hybridization case study},
  author={F. J. Burpo},
  • F. J. Burpo
  • Published 2001
  • Computer Science
  • The success of the polymerase chain reaction (PCR) is highly dependent on primer design. Commonly used primer design programs rely upon a core set of parameters such as melting temperature, primer length, GC content, and complementarity to optimize the PCR product, but weight those parameters to differing degrees, as well as include other parameters for PCR specific tasks. An analysis of these design algorithms ,and other available PCR primer analysis software, was conducted to find the best… CONTINUE READING
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