A critical evaluation of in situ measurement of mitochondrial electrical potentials in single hepatocytes.

@article{Ubl1996ACE,
  title={A critical evaluation of in situ measurement of mitochondrial electrical potentials in single hepatocytes.},
  author={J J Ubl and Jean-Yves Chatton and S Chen and J{\"o}rg W Stucki},
  journal={Biochimica et biophysica acta},
  year={1996},
  volume={1276 2},
  pages={124-32}
}
The range of applicability and the critical parameters involved in the assessment of mitochondrial electrical potential (delta psi mit) using epifluorescence microscopy were evaluated based on both theoretical and experimental analysis. Rat hepatocytes loaded with the potential-dependent fluorescent dye rhodamine 123 exhibited the expected heterogeneity of fluorescence distribution with dark regions corresponding to the nucleus and bright regions corresponding to the mitochondria-rich cytosol… CONTINUE READING
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