A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells

  title={A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells},
  author={Soichiro Ogaki and Mayu Morooka and Kaito Otera and Shoen Kume},
  journal={Scientific Reports},
The human intestinal epithelium is a useful model for pharmacological studies of absorption, metabolism, drug interactions, and toxicology, as well as for studies of developmental biology. We established a rapid and cost effective system for differentiation of human induced pluripotent stem (iPS) cells into definitive endoderm (DE) cells. In the presence of dimethyl sulfoxide (DMSO), a low concentration of Activin at 6.25 ng/ml is sufficient to give a similar differentiation efficiency with… 

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