A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells
@article{Ogaki2015ACS, title={A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells}, author={Soichiro Ogaki and Mayu Morooka and Kaito Otera and Shoen Kume}, journal={Scientific Reports}, year={2015}, volume={5} }
The human intestinal epithelium is a useful model for pharmacological studies of absorption, metabolism, drug interactions, and toxicology, as well as for studies of developmental biology. We established a rapid and cost effective system for differentiation of human induced pluripotent stem (iPS) cells into definitive endoderm (DE) cells. In the presence of dimethyl sulfoxide (DMSO), a low concentration of Activin at 6.25 ng/ml is sufficient to give a similar differentiation efficiency with…
35 Citations
Establishment of a novel culture method for maintaining intestinal stem cells derived from human induced pluripotent stem cells
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A novel two-dimensional culture method for maintaining intestinal stem cells (ISCs) derived from human iPS cells with differentiation potency is established and differentiated enterocytes differentiated from maintained ISCs showed pharmacokinetics functions.
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This work compared maturation of 2D-cultured iPCSs toward small intestinal epithelial cells when cultured either with or without serum, and either on Geltrex or on animal-free, recombinant laminin-based substrata, and suggested that differentiation toward definitive endoderm is more efficient without serum.
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Pharmacokinetic functions of human induced pluripotent stem cell-derived small intestinal epithelial cells.
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To develop an efficient differentiation method to induce endoderm formation from human iPSCs, the differentiated HiEnts and HiHeps with pharmacokinetic functions could be used for drug development.
Retinoic acid promotes barrier functions in human iPSC-derived intestinal epithelial monolayers.
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Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation.
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The DMSO pretreatment improves PSC differentiation by regulating the cell cycle and priming stem cells to be more responsive to differentiation signals, resulting in more efficiently differentiate PSCs to any lineage of choice.
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