A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells

@article{Ogaki2015ACS,
  title={A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells},
  author={Soichiro Ogaki and Mayu Morooka and Kaito Otera and Shoen Kume},
  journal={Scientific Reports},
  year={2015},
  volume={5}
}
The human intestinal epithelium is a useful model for pharmacological studies of absorption, metabolism, drug interactions, and toxicology, as well as for studies of developmental biology. We established a rapid and cost effective system for differentiation of human induced pluripotent stem (iPS) cells into definitive endoderm (DE) cells. In the presence of dimethyl sulfoxide (DMSO), a low concentration of Activin at 6.25 ng/ml is sufficient to give a similar differentiation efficiency with… 

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Cyclic AMP Signaling Promotes the Differentiation of Human Induced Pluripotent Stem Cells into Intestinal Epithelial Cells

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References

SHOWING 1-10 OF 51 REFERENCES

Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro

A robust and efficient process is established to direct the differentiation of human PSCs into intestinal tissue in vitro using a temporal series of growth factor manipulations to mimic embryonic intestinal development and indicates that human intestinal stem cells form de novo during development.

An in vivo model of human small intestine using pluripotent stem cells

This model of the human small intestine may pave the way for studies of intestinal physiology, disease and translational studies and a role for circulating factors in the intestinal adaptive response is suggested.

A synthetic nanofibrillar matrix promotes in vitro hepatic differentiation of embryonic stem cells and induced pluripotent stem cells

It is suggested that morphological changes result in cellular differentiation controlled by Rac1 activation, and that motility is not only the consequence, but is also able to trigger differentiation.

Generation of anterior foregut endoderm from human embryonic and induced pluripotent stem cells

It is found that dual inhibition of transforming growth factor (TGF)-β and bone morphogenic protein (BMP) signaling after specification of definitive endoderm from pluripotent cells results in a highly enriched AFE population that is competent to be patterned along dorsoventral and anteroposterior axes.

Generation of insulin-producing β-like cells from human iPS cells in a defined and completely xeno-free culture system.

The results demonstrate the feasibility of generating hiPS-derived pancreatic β-cells under xeno-free conditions and highlight their potential to treat patients with type 1 diabetes.

The effects of solvents on embryonic stem cell differentiation.

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