A correlation between soluble brain nitric oxide synthase and NADPH-diaphorase activity is only seen after exposure of the tissue to fixative.

@article{Matsumoto1993ACB,
  title={A correlation between soluble brain nitric oxide synthase and NADPH-diaphorase activity is only seen after exposure of the tissue to fixative.},
  author={Takeshi Matsumoto and Masaki Nakane and Jennifer S. Pollock and Jane Kuk and Ulrich Foerstermann},
  journal={Neuroscience letters},
  year={1993},
  volume={155 1},
  pages={61-4}
}
In histochemical studies using fixed brain tissue, NADPH-diaphorase has been found to be colocalized with soluble nitric oxide synthase. In the present study, using fresh tissues from eight different regions of rat brain, NADPH-diaphorase activity was found mostly in the particulate fraction, whereas most of the nitric oxide synthase activity was located to the cytosolic fraction. Also, the distribution of NADPH-diaphorase activity among brain regions was different from that of nitric oxide… CONTINUE READING

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