A convenient spectrophotometric assay for the determination of L-ergothioneine in blood.

@article{Carlsson1974ACS,
  title={A convenient spectrophotometric assay for the determination of L-ergothioneine in blood.},
  author={Jan Carlsson and M. P. J. Kierstan and Keith Brocklehurst},
  journal={The Biochemical journal},
  year={1974},
  volume={139 1},
  pages={237-42}
}
1. A convenient spectrophotometric assay for the determination of l-ergothioneine in solution including deproteinized blood haemolysate was developed. 2. The method consists of deproteinization by heat precipitation and Cu(2+)-catalysed oxidation of thiols such as glutathione and of l-ascorbic acid, both in alkaline media, and titration of l-ergothioneine (which is not oxidized under these conditions) by its virtually instantaneous reaction with 2,2'-dipyridyl disulphide at pH1. 3. This method… CONTINUE READING

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