A convenient sandwich assay of thrombin in biological media using nanoparticle-enhanced fluorescence polarization.

Abstract

A new aptamer biosensor was presented for the detection of thrombin in this work, which was based on fluorescence polarization (FP) using silica nanoparticles as enhancement probe. The silica nanoparticles covered by streptavidin were tagged with a thrombin aptamer (5'-biotin-GGTTGGTGTGGTTGG-3'), which was bound to the surface of silica nanoparticle through… (More)
DOI: 10.1016/j.bios.2014.01.021

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