A convenient rapid culture assay for the detection of enteroviruses in clinical samples: comparison with conventional cell culture and RT-PCR.

@article{TerletskaiaLadwig2008ACR,
  title={A convenient rapid culture assay for the detection of enteroviruses in clinical samples: comparison with conventional cell culture and RT-PCR.},
  author={Elena Terletskaia-Ladwig and Silvia Meier and Ralph Hahn and Michael Leinm{\"u}ller and Franz Anton Schneider and M Enders},
  journal={Journal of medical microbiology},
  year={2008},
  volume={57 Pt 8},
  pages={1000-6}
}
A convenient rapid culture assay (RCA) for the detection of enteroviruses was evaluated against RT-PCR using 576 stool and 102 cerebrospinal fluid (CSF) samples. One hundred and ninety stool samples were also tested by conventional cell culture (CCC). The RCA used immunoperoxidase staining of cell culture plates with a blend of monoclonal antibodies (mAbs) against enterovirus VP1 on the second and sixth days after inoculation. This blend was composed of 5D8/1 (Dako) and four 'in-house' mAbs… CONTINUE READING

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