A convenient in vitro screening method for predicting in vivo drug metabolic clearance using isolated hepatocytes suspended in serum.

@article{Shibata2000ACI,
  title={A convenient in vitro screening method for predicting in vivo drug metabolic clearance using isolated hepatocytes suspended in serum.},
  author={Yoshihiro Shibata and Harumi Takahashi and Yasuyuki Ishii},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2000},
  volume={28 12},
  pages={1518-23}
}
A novel and convenient in vitro method for predicting in vivo metabolic clearance in the liver (CL(H)) was developed. The CL(H) of a drug is usually predicted by using both the unbound fraction in serum and the intrinsic hepatic clearance of the unbound fraction, but this procedure is labor-intensive. We simplified the method by directly measuring intrinsic hepatic clearance using isolated rat hepatocytes suspended in rat serum and called this "the serum incubation method". Sixteen commercially… CONTINUE READING
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