A convenient homogeneous enzyme immunoassay for estradiol detection

@article{Chiu2011ACH,
  title={A convenient homogeneous enzyme immunoassay for estradiol detection},
  author={May L. Chiu and Tina T.-C. Tseng and Harold G. Monbouquette},
  journal={Biotechnology and Applied Biochemistry},
  year={2011},
  volume={58}
}
A convenient homogeneous enzyme immunoassay for estradiol is described. Unlike heterogeneous immunoassays, which require time‐consuming separation steps or expensive automated systems, homogeneous immunoassays, wherein all reagents are freely suspended in bulk solution, can be simple and fast without costly instrumentation. The key component of this assay system, an estradiol–reporter enzyme conjugate, was prepared by covalently binding β‐estradiol‐6‐(O‐carboxymethyl)oxime to glucose‐6… 
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