A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase.

@article{Bowers1966ACS,
  title={A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase.},
  author={G. Bowers and R. Mccomb},
  journal={Clinical chemistry},
  year={1966},
  volume={12 2},
  pages={
          70-89
        }
}
A continuous spectrophotometric method for measuring serum alkaline phosphatase activity is described. The effects of temperature, pH, substrate concentration, type and molarity of the buffer, sample size, cofactors, and inhibitors on the enzymatic hydrolysis of p-nitrophenyl phosphate were studied. The optimal conditions for assay of serum alkaline phosphatase at 30° were found to be 0.75 M 2-amino-2-methyl-1-propanol buffer, pH30° 10.15, 4 mmole substrate, and 100 µl. or less sample size… Expand
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