A continuous fluorescence assay for the characterization of Nudix hydrolases

Abstract

The common substrate structure for the functionally diverse Nudix protein superfamily is nucleotide-diphosphate-X, where X is a large variety of leaving groups. The substrate specificity is known for less than 1% of the 29,400 known members. Most activities result in the release of an inorganic phosphate ion or of a product bearing a terminal phosphate… (More)
DOI: 10.1016/j.ab.2013.02.023

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