A continuous flow technique for analysis of the stoichiometry of the gastric H,K-ATPase.

A continuous flow method was developed for determining the stoichiometry of the gastric proton pump H,K-ATPase in its hydrolysis of ATP, translocation of H+ and the K+ congener 86Rb+. H,K-ATPase-containing vesicles which had been isolated from pig gastric mucosa were incubated at 37 degrees C for 2 h in 150 mM 86RbCl, 0.5 mM EGTA and 3 mM Mes-buffer… CONTINUE READING