A conserved upstream element is essential for transcription of the Leishmania tarentolae mini-exon gene.

@article{Saito1994ACU,
  title={A conserved upstream element is essential for transcription of the Leishmania tarentolae mini-exon gene.},
  author={Rosa M. Saito and Marc G. Elgort and David A. Campbell},
  journal={The EMBO journal},
  year={1994},
  volume={13 22},
  pages={5460-9}
}
We demonstrate that the mini-exon genes of Leishmania tarentolae are individually transcribed by an enzyme pharmacologically identified as RNA polymerase II. To study transcription in these ancient eukaryotes, a stable transformation assay using an episomal mini-exon gene was developed. The introduced mini-exon gene, which had been marked with a 40 bp tag, yielded the predicted tagged transcript. An upstream cis-acting element that was essential for transcription of the mini-exon gene was… CONTINUE READING