A conserved function for pericentromeric satellite DNA

@article{Jagannathan2018ACF,
  title={A conserved function for pericentromeric satellite DNA},
  author={Madhav Jagannathan and Ryan Cummings and Yukiko M. Yamashita},
  journal={eLife},
  year={2018},
  volume={7}
}
A universal and unquestioned characteristic of eukaryotic cells is that the genome is divided into multiple chromosomes and encapsulated in a single nucleus. However, the underlying mechanism to ensure such a configuration is unknown. Here we provide evidence that pericentromeric satellite DNA, which is often regarded as junk, is a critical constituent of the chromosome, allowing the packaging of all chromosomes into a single nucleus. We show that the multi AT-hook satellite DNA binding… 

The modular mechanism of chromocenter formation in Drosophila

It is shown that chromocenter formation is mediated by a ‘modular’ network, where interactions between two sequence-specific satellite DNA-binding proteins, D1 and Prod, bound to their cognate satellite DNAs, bring the full complement of chromosomes into the Chromocenter.

Defective Satellite DNA Clustering into Chromocenters Underlies Hybrid Incompatibility in Drosophila

It is shown that ineffective clustering of divergent satellite DNA in the cells of Drosophila hybrids results in chromocenter disruption, associated micronuclei formation and tissue atrophy, and is proposed a unifying framework that explains how the widely observed satellite DNA divergence between closely related species can cause reproductive isolation.

Functional Significance of Satellite DNAs: Insights From Drosophila

This review summarizes the current knowledge concerning satellite functions, the mechanisms of regulation and evolution of satellites, focusing on recent findings in Drosophila, suggesting relevance of the analysis to a wide range of eukaryotic organisms.

Centromere Repeats: Hidden Gems of the Genome

The regulatory activity of satellite DNAs and their neighboring transposable elements in a chromosomal context with a particular emphasis on the integral role of both in centromere function is discussed, to disparage the conventional exemplification of repetitive DNA in the historically-associated context of ‘junk’.

Cross-species incompatibility between a DNA satellite and the Drosophila Spartan homolog poisons germline genome integrity

The observed 359-MH[sim] cross-species incompatibility supports a model under which ostensibly inert repetitive DNA and essential chromosomal proteins must coevolve to preserve germline genome integrity.

Pericentromeric heterochromatin impacts genome compartmentalization and sex chromosome evolution in a fish

It is demonstrated that the pericentromeric heterochromatin (PCH) can cover a considerably large portion of the chromosomes, and when it does so, it drives chromosome compartmentalization; and a new model for the origin and evolution of homomorphic sex chromosomes in fish is proposed.

Sequence, Chromatin and Evolution of Satellite DNA

Different classes of satellite DNA sequences, their satellite-specific chromatin features, and how these features may contribute to satellite DNA biology and evolution are described.

The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila

A distinct six subunit protein complex containing HMR and LHR is described and the effect of Hmr mutations on complex integrity and function is analysed, suggesting that HMR needs to bring together components of centromeric and pericentromeric chromatin to fulfil its physiological function and to cause hybrid male lethality.
...

References

SHOWING 1-10 OF 88 REFERENCES

Heterochromatin, Satellite DNA, and Cell Function

It is concluded that a certain amount of constitutive heterochromatin is essential in multicellular organisms at two levels of organization, chromosomal and nuclear.

Heterochromatin, satellite DNA, and cell function. Structural DNA of eucaryotes may support and protect genes and aid in speciation.

It is concluded that a certain amount of constitutive heterochromatin is essential in multicellular organisms at two levels of organization, chromosomal and nuclear.

The molecular basis of the organization of repetitive DNA-containing constitutive heterochromatin in mammals

Ch Chromatin immunoprecipitation followed by sequencing (ChIP-seq) analysis is a powerful tool to investigate the epigenetic regulation of eukaryote genomes, but non-unique reads are usually discarded during standard ChIP- sequencing data alignment to reference genome databases, so specific methods to obtain global epigenetic information concerning repetitive elements are needed.

Molecular Structure of a Functional Drosophila Centromere

Mouse centric and pericentric satellite repeats form distinct functional heterochromatin

It is shown that centric and pericentric repeats on the chromosomes have distinct heterochromatic characteristics in the nucleus, and spatio-temporal isolation is proposed to be important for centromeric cohesion and dissociation during chromosome segregation.

Interphase chromosomes in Arabidopsis are organized as well defined chromocenters from which euchromatin loops emanate

The arrangement of interphase chromosomes in Arabidopsis provides a well defined system to investigate chromatin organization and its role in epigenetic processes.

DNA breaks and chromosome pulverization from errors in mitosis

A mechanism by which errors in mitotic chromosome segregation generate DNA breaks via the formation of structures called micronuclei is identified, which potentially lead to mutations and chromosome rearrangements that can integrate into the genome.

Sequence analysis of a functional Drosophila centromere.

The results of extensive sequence analysis of a fully functional centromere present in the Drosophila Dp1187 minichromosome demonstrate that the sequence composition and organization of large regions of centric heterochromatin can be determined, despite the presence of repeated DNA.
...