A conserved cis-acting sequence in the 5' leader of avian sarcoma virus RNA is required for packaging.

@article{Katz1986ACC,
  title={A conserved cis-acting sequence in the 5' leader of avian sarcoma virus RNA is required for packaging.},
  author={Richard A. Katz and Robert W. Terry and Anna Marie Skalka},
  journal={Journal of virology},
  year={1986},
  volume={59 1},
  pages={
          163-7
        }
}
The deletion of a conserved sequence of ca. 30 nucleotides in the 5' noncoding leader region of an avian sarcoma virus DNA clone resulted in a loss of infectivity after transfection of chicken embryo fibroblasts. Genetic and biochemical analysis of a representative mutant demonstrated that the env gene was expressed normally. Thus, viral RNA transcription, splicing, and translation were not impaired. The amount of mutant viral RNA encapsidated into virions, however, was severely reduced despite… CONTINUE READING

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