A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation

@article{Shaw1986ACA,
  title={A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation},
  author={Gray D. Shaw and Robert I. Kamen},
  journal={Cell},
  year={1986},
  volume={46},
  pages={659-667}
}
The mRNAs of transiently expressed genes frequently contain an AU-rich sequence in the 3' untranslated region. We introduced a 51 nucleotide AT sequence from a human lymphokine gene, GM-CSF, into the 3' untranslated region of the rabbit beta-globin gene. Our experiments demonstrate that this caused the otherwise stable beta-globin mRNA to become highly unstable in vivo. The instability conferred by the AU sequence in the mRNA was partially alleviated by treatment of the cells with cycloheximide… 
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It is proposed that RNA instability mediated by the AU motif is achieved through translation-dependent assembly of this large mRNA-destabilizing complex found only on unstable RNAs.
Translational blockade imposed by cytokine-derived UA-rich sequences.
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The messenger RNAs specifying certain proteins involved in the inflammatory response and certain oncoproteins contain a conserved UA-rich sequence in the 3' untranslated region, which has been shown to destabilize mRNA in some eukaryotes.
Identification of AU‐Rich 3’ Untranslated Regions in mRNA from Sea Urchin Coelomocytes
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The conservation of 3' AURE in functionally related mammalian proteins and their potential importance in regulating the expression of proteins that govern the inflammatory response, wound healing, cell adhesion, and cell growth suggested to us that these elements may have been highly conserved throughout evolution.
Identification of a translation inhibitory element (TIE) in the 3' untranslated region of the human interferon-beta mRNA.
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The removal of the translation inhibitory sequence not only improves the mRNA translation in Xenopus oocytes but it also strongly decreases the IFN-beta mRNA stability in those cells, suggesting that, in this system at least, the mRNA degradation is linked to its translational efficiency.
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    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
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