A connectome and analysis of the adult Drosophila central brain

@article{Scheffer2020ACA,
  title={A connectome and analysis of the adult Drosophila central brain},
  author={Louis K. Scheffer and C. Shan Xu and Michał Januszewski and Zhiyuan Lu and Shin-ya Takemura and Kenneth J. Hayworth and Gary B. Huang and Kazunori Shinomiya and Jeremy B. Maitin-Shepard and Stuart E. Berg and Jody Clements and Philip M. Hubbard and William T. Katz and Lowell A. Umayam and Ting Zhao and David Ackerman and Tim Blakely and John A. Bogovic and Tom Dolafi and Dagmar Kainmueller and Takashi Kawase and Khaled A. Khairy and Laramie Leavitt and Peter H. Li and Larry F. Lindsey and Nicole L. Neubarth and Donald J. Olbris and Hideo Otsuna and Eric T. Trautman and Masayoshi Ito and Jens Goldammer and Tanya Wolff and Robert R. Svirskas and Philipp Schlegel and Erika Rae Neace and Christopher J Knecht and Chelsea X. Alvarado and Dennis A Bailey and Samantha Ballinger and Jolanta A. Borycz and Brandon S Canino and Natasha Cheatham and Michael Cook and Marisa Dreher and Octave Duclos and Bryon Eubanks and Kelli Fairbanks and Samantha Finley and Nora Forknall and Audrey Francis and Gary Patrick Hopkins and Emily M Joyce and SungJin Kim and Nicole A. Kirk and Julie Kovalyak and Shirley Lauchie and Alanna Lohff and Charli Maldonado and Emily A. Manley and Sari McLin and Caroline Mooney and Miatta Ndama and Omotara Ogundeyi and Nneoma Okeoma and Christopher Ordish and Nicholas Padilla and Christopher M Patrick and Tyler Paterson and Elliott E Phillips and Emily M. Phillips and Neha Rampally and Caitlin Ribeiro and Madelaine K Robertson and Jon Thomson Rymer and Sean Michael Ryan and Megan Sammons and Anne K. Scott and Ashley L Scott and Aya Shinomiya and Claire Smith and Kelsey Smith and Natalie L Smith and Margaret A. Sobeski and Alia Suleiman and Jackie Swift and Satoko Takemura and Iris Talebi and Dorota Tarnogorska and Emily Tenshaw and Temour Tokhi and John J. Walsh and Tansy Yang and Jane Anne Horne and Feng Li and Ruchi Parekh and Patricia K. Rivlin and Vivek Jayaraman and Kei Ito and Stephan Saalfeld and Reed A. George and Ian A. Meinertzhagen and Gerald M. Rubin and Harald F. Hess and Viren Jain and Stephen M. Plaza},
  journal={eLife},
  year={2020},
  volume={9}
}
The neural circuits responsible for animal behavior remain largely unknown. We summarize new methods and present the circuitry of a large fraction of the brain of the fruit fly Drosophila melanogaster. Improved methods include new procedures to prepare, image, align, segment, find synapses in, and proofread such large data sets. We define cell types, refine computational compartments, and provide an exhaustive atlas of cell examples and types, many of them novel. We provide detailed circuits… 
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References

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A Connectome of the Adult Drosophila Central Brain
TLDR
New methods are summarized and the complete circuitry of a large fraction of the brain of a much more complex animal, the fruit fly Drosophila melanogaster, is presented, reducing the effort needed to answer typical circuit questions.
The connectome of the adult Drosophila mushroom body: implications for function
TLDR
This work identifies new components of the MB circuit in Drosophila, including extensive visual input and MB output neurons (MBONs) with direct connections to descending neurons, and provides insights into the circuitry used to integrate MB outputs, connectivity between the MB and the central complex and inputs to DANs, including feedback from MBONs.
Connectome studies on Drosophila: a short perspective on a tiny brain
TLDR
Attempts in Drosophila anticipate parallel attempts in other systems, notably the inner plexiform layer of the vertebrate retina, and augment the one complete connectome already available to us, that available for 30 years in the nematode Caenorhabditis elegans.
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The results show that the fly nerve cord is a highly organized, layered system of neuropils, an organization that reflects the fact that insects are capable of two largely independent means of locomotion – walking and fight – using distinct sets of appendages.
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An updated map of the Drosophila central complex will facilitate a deeper behavioral and physiological dissection of this sophisticated set of structures and provides new insights into the anatomical structure of the four components of the central complex and its accessory neuropils.
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This work uses morphological and electrophysiological data to deduce the passive membrane properties of antennal lobe projection neurons and to build a compartmental model of their electrotonic structure, finding that these neurons are electrotonically extensive and that a somatic recording electrode can only imperfectly control the voltage in the rest of the cell.
Automatic Neuron Type Identification by Neurite Localization in the Drosophila Medulla
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This work introduces algorithms to cluster similarly-shaped neurons, where 3D neuronal shapes are represented as skeletons, and proposes a novel location-sensitive clustering algorithm that shows clustering results on neurons reconstructed from the Drosophila medulla that show high-accuracy.
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