A conformational change in cytochrome c of apoptotic and necrotic cells is detected by monoclonal antibody binding and mimicked by association of the native antigen with synthetic phospholipid vesicles.

@article{Jemmerson1999ACC,
  title={A conformational change in cytochrome c of apoptotic and necrotic cells is detected by monoclonal antibody binding and mimicked by association of the native antigen with synthetic phospholipid vesicles.},
  author={Ronald Jemmerson and Jun Liu and Danielle L Hausauer and Kong Peng Lam and Anna Mondino and R Darrell Nelson},
  journal={Biochemistry},
  year={1999},
  volume={38 12},
  pages={3599-609}
}
By flow cytometry, a conformational change in mouse cytochrome c (cyt c) of apoptotic and necrotic T hybridoma cells was detected using a monoclonal antibody (mAb) that recognizes the region around amino acid residue 44 on a non-native form of the protein. The conformational change in cyt c is an early event in apoptosis, which can be identified in pre-apoptotic cells that are negative for other indicators of apoptosis. Since the mAb did not bind fixed and permeabilized live cells and did not… CONTINUE READING
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