A comprehensive two-dimensional map of cytosolic proteins of Bacillus subtilis.

@article{Buettner2001ACT,
  title={A comprehensive two-dimensional map of cytosolic proteins of Bacillus subtilis.},
  author={Knut Buettner and J{\"o}rg Bernhardt and Christian Scharf and Roland Schmid and Ulrike M{\"a}der and Christine Eymann and Haike Antelmann and Andrea V{\"o}lker and Uwe Voelker and Michael Hecker},
  journal={Electrophoresis},
  year={2001},
  volume={22 14},
  pages={2908-35}
}
Proteomics relying on two-dimensional (2-D) gel electrophoresis of proteins followed by spot identification with mass spectrometry is an excellent experimental tool for physiological studies opening a new perspective for understanding overall cell physiology. This is the intriguing outcome of a method introduced by Klose and O'Farrell independently 25 years ago. Physiological proteomics requires a 2-D reference map on which most of the main proteins were identified. In this paper, we present… CONTINUE READING