A comprehensive method for extraction and quantitative analysis of sterols and secosteroids from human plasma.

@article{McDonald2012ACM,
  title={A comprehensive method for extraction and quantitative analysis of sterols and secosteroids from human plasma.},
  author={Jeffrey G McDonald and Daniel D Smith and Ashlee R Stiles and D. W. Russell},
  journal={Journal of lipid research},
  year={2012},
  volume={53 7},
  pages={1399-409}
}
We describe the development of a method for the extraction and analysis of 62 sterols, oxysterols, and secosteroids from human plasma using a combination of HPLC-MS and GC-MS. Deuterated standards are added to 200 μl of human plasma. Bulk lipids are extracted with methanol:dichloromethane, the sample is hydrolyzed using a novel procedure, and sterols and secosteroids are isolated using solid-phase extraction (SPE). Compounds are resolved on C₁₈ core-shell HPLC columns and by GC. Sterols and… CONTINUE READING

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DRI Dietary Reference of Calcium and Vitamin D, in Dietary Reference Intakes

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  • Institute of Medicine of the National Academies:
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