A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae

@article{Uetz2000ACA,
  title={A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae},
  author={P. Uetz and Loic Giot and Gerard Cagney and Traci A. Mansfield and Richard S. Judson and James R. Knight and Daniel Lockshon and Vaibhav A. Narayan and Maithreyan Srinivasan and P Pochart and Alia Qureshi-Emili and Youhai Li and Brian Christopher Godwin and D Conover and Theodore S Kalbfleisch and Govindan Vijayadamodar and Meijia Yang and Mark Johnston and Stanley Fields and Jonathan Rothberg},
  journal={Nature},
  year={2000},
  volume={403},
  pages={623-627}
}
Two large-scale yeast two-hybrid screens were undertaken to identify protein–protein interactions between full-length open reading frames predicted from the Saccharomyces cerevisiae genome sequence. In one approach, we constructed a protein array of about 6,000 yeast transformants, with each transformant expressing one of the open reading frames as a fusion to an activation domain. This array was screened by a simple and automated procedure for 192 yeast proteins, with positive responses… 
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TLDR
An efficient two-hybrid strategy to facilitate construction of protein interaction maps on a genome-wide scale that has several advantages over previously described approaches, including the ability to detect interactions with proteins that inhibit yeast growth or that activate transcription as BD fusions.
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