A complete bacterial genome assembled de novo using only nanopore sequencing data

@article{Loman2015ACB,
  title={A complete bacterial genome assembled de novo using only nanopore sequencing data},
  author={Nicholas J. Loman and Joshua Quick and Jared T. Simpson},
  journal={Nature Methods},
  year={2015},
  volume={12},
  pages={733-735}
}
We have assembled de novo the Escherichia coli K-12 MG1655 chromosome in a single 4.6-Mb contig using only nanopore data. Our method has three stages: (i) overlaps are detected between reads and then corrected by a multiple-alignment process; (ii) corrected reads are assembled using the Celera Assembler; and (iii) the assembly is polished using a probabilistic model of the signal-level data. The assembly reconstructs gene order and has 99.5% nucleotide identity. 
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