A competitive enzyme-linked immunosorbent assay to quantitate acyclovir and BW B759U in human plasma and urine.

@article{Tadepalli1986ACE,
  title={A competitive enzyme-linked immunosorbent assay to quantitate acyclovir and BW B759U in human plasma and urine.},
  author={S M Tadepalli and Robert P. Quinn and Devron R Averett},
  journal={Antimicrobial agents and chemotherapy},
  year={1986},
  volume={29 1},
  pages={93-8}
}
A simple and sensitive enzyme-linked immunosorbent assay for the detection and quantitation of acyclovir in human plasma and urine was developed. Acyclovir immobilized on a solid phase and free acyclovir in the sample solution were allowed to compete for a limited amount of anti-acyclovir monoclonal antibody. The specific antibody bound to the immobilized acyclovir was detected by the use of alkaline phosphatase-conjugated anti-mouse immunoglobulin. The resulting enzyme activity was inversely… CONTINUE READING