A comparison of thiosulphate‐citrate‐bile salts‐sucrose (TCBS) agar and thiosulphate‐chloride‐iodide (TCI) agar for the isolation of Vibrio species from estuarine environments

  title={A comparison of thiosulphate‐citrate‐bile salts‐sucrose (TCBS) agar and thiosulphate‐chloride‐iodide (TCI) agar for the isolation of Vibrio species from estuarine environments},
  author={C. Pfeffer and James Douglas Oliver},
  journal={Letters in Applied Microbiology},
Aims: The purpose of this study was to compare a recently described medium, thiosulphate‐chloride‐iodide (TCI), for the isolation of estuarine vibrios with thiosulphate‐citrate‐bile salts‐sucrose (TCBS). 
Identification of epiphytic bacterial communities associated with the brown alga Splachnidium rugosum
The composition and uniqueness of epiphytic bacterial communities associated with S. rugosum are reported on, with Gamma-Proteobacteria were the most abundant, dominated by Vibrio and Pseudoalteromonas species.
Antagonistic activity of marine Streptomyces sp. S073 on pathogenic Vibrio parahaemolyticus
Marine Streptomyces sp. isolate S073 was shown to have strong antagonistic activity towards the pathogenic Vibrio parahaemolyticus using the agar diffusion method. The antagonistic substance(s)
Identification of a chromosomally-encoded sucrose operon-like gene cluster in Vibrio parahaemolyticus strain PH05 isolated from Negros Island, Philippines
The ability of bacteria to metabolize a wide variety of carbon sources has been known to aid in their ability for efficient colonization. Vibrio parahaemolyticus, a known aquatic pathogen has been
Inhibition of bacterial growth using false yam (Icacina oliviformis) extract as an additive in liquid soap
False yam (Icacina oliviformis) is a drought-resistant root crop found in Northern Ghana. Research confirms it to contain some levels of antimicrobial, antifungal, antidiarrheal, antioxidant, and
Development of enhanced selective media for detection of Vibrio parahaemolyticus in oysters
TSAO SS1 with high selectivity potentials over TCBS may be a promising alternative for detection of V .
Vibrio cholerae in the Environment: A Simple Method for Reliable Identification of the Species
It was demonstrated here that the yellow colonies (sucrose-fermenting), which are able to grow on nutrient agar without added NaCl and which present a positive oxidase reaction, can be confidently considered as presumptive V. cholerae.
Interaction of Vibrio to Biotic and Abiotic Surfaces: Relationship between Hydrophobicity, Cell Adherence, Biofilm Production, and Cytotoxic Activity
Vibrio parahaemolyticus and Vibrio alginolyticus are important pathogenic agents for both humans and aquatic animals. Twenty-five bacterial strains were isolated from infected sea bass (Dicentrarchus
Genetic diversity and phenotypic plasticity of AHL-mediated Quorum sensing in environmental strains of Vibrio mediterranei
The results suggest that isolates of a single genotype are able to change their QS phenotypes in response to environmental conditions, highlighting the phenotypic plasticity of bacterial communication in the environment.
The Bacterial (Vibrio alginolyticus) Production of Tetrodotoxin in the Ribbon Worm Lineus longissimus—Just a False Positive?
Test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production suggest that re-examination of some published claims of TTXProduction may be warranted.
In vitro and in vivo potential probiotic activity of Bacillus subtilis and Shewanella algae for use in Litopenaeus vannamei rearing
Investigation of the antimicrobial activity of three candidate probiotic strains showed that B. subtilis strain administration significantly improved shrimp growth and could develop in shrimp hepatopancreas and intestine.


Thiosulfate-citrate-bile salts-sucrose agar and its selectivity for clinical and marine vibrio organisms.
TCBS grew vibrios well with distinctive morphology, however, its limited selectivity needs to be more widely appreciated in clinical laboratories.
Statistical evaluation of a quality control method for isolation of pathogenic Vibrio species on selected thiosulfate-citrate-bile salts-sucrose agars
The recovery and quality of growth of each species varied significantly on the different brands of TCBS agar, and significant variability was also identified for some components of the inoculation procedure.
Culture media for the isolation and enumeration of pathogenic Vibrio species in foods and environmental samples.
A wide range of enrichment and selective media for the isolation of vibrios has been developed and media are reviewed with respect to their ability to recover and differentiate the target vibrio.
Evaluation of selective media for isolation and enumeration of vibrios from estuarine waters
The TCBS is considered as the best medium to isolate Vibrio species pathogenic for humans and fish, and for recovery of vibrios from estuarine waters.
Chapter 17 Culture media for the isolation and enumeration of pathogenic Vibrio species in foods and environmental samples
Alkaline peptone water (APW) and thiosulphate citrate bile salts sucrose agar are the most widely employed for the enrichment and isolation of V. cholerae and V. vulnificus, and cellobiose polymyxin colistin agar is the most commonly used selective medium.
TCI – a new bile free medium for the isolation of Vibrio species
  • Australian Journal of Medical Science
  • 1992
Evaluation of a new selective agar, thiosulfate-chloride-iodide (TCI), for the growth of pathogenic Vibrio species
  • Medical Microbiology Letters
  • 1993
Media for Vibrio species
  • Quality Assurance and Quality Control of Microbiological Culture Media ed
  • 1982
A comparison of media for the isolation of Vibrio spp. from environmental sources
  • Abstracts of the Annual Meeting of the American Society for Microbiology
  • 1985