A comparison of thiosulphate‐citrate‐bile salts‐sucrose (TCBS) agar and thiosulphate‐chloride‐iodide (TCI) agar for the isolation of Vibrio species from estuarine environments

@article{Pfeffer2003ACO,
  title={A comparison of thiosulphate‐citrate‐bile salts‐sucrose (TCBS) agar and thiosulphate‐chloride‐iodide (TCI) agar for the isolation of Vibrio species from estuarine environments},
  author={C. Pfeffer and James Douglas Oliver},
  journal={Letters in Applied Microbiology},
  year={2003},
  volume={36}
}
Aims: The purpose of this study was to compare a recently described medium, thiosulphate‐chloride‐iodide (TCI), for the isolation of estuarine vibrios with thiosulphate‐citrate‐bile salts‐sucrose (TCBS). 
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References

SHOWING 1-9 OF 9 REFERENCES
Thiosulfate-citrate-bile salts-sucrose agar and its selectivity for clinical and marine vibrio organisms.
TLDR
TCBS grew vibrios well with distinctive morphology, however, its limited selectivity needs to be more widely appreciated in clinical laboratories.
Statistical evaluation of a quality control method for isolation of pathogenic Vibrio species on selected thiosulfate-citrate-bile salts-sucrose agars
TLDR
The recovery and quality of growth of each species varied significantly on the different brands of TCBS agar, and significant variability was also identified for some components of the inoculation procedure.
Culture media for the isolation and enumeration of pathogenic Vibrio species in foods and environmental samples.
TLDR
A wide range of enrichment and selective media for the isolation of vibrios has been developed and media are reviewed with respect to their ability to recover and differentiate the target vibrio.
Evaluation of selective media for isolation and enumeration of vibrios from estuarine waters
TLDR
The TCBS is considered as the best medium to isolate Vibrio species pathogenic for humans and fish, and for recovery of vibrios from estuarine waters.
Chapter 17 Culture media for the isolation and enumeration of pathogenic Vibrio species in foods and environmental samples
TLDR
Alkaline peptone water (APW) and thiosulphate citrate bile salts sucrose agar are the most widely employed for the enrichment and isolation of V. cholerae and V. vulnificus, and cellobiose polymyxin colistin agar is the most commonly used selective medium.
TCI – a new bile free medium for the isolation of Vibrio species
  • Australian Journal of Medical Science
  • 1992
Evaluation of a new selective agar, thiosulfate-chloride-iodide (TCI), for the growth of pathogenic Vibrio species
  • Medical Microbiology Letters
  • 1993
Media for Vibrio species
  • Quality Assurance and Quality Control of Microbiological Culture Media ed
  • 1982
A comparison of media for the isolation of Vibrio spp. from environmental sources
  • Abstracts of the Annual Meeting of the American Society for Microbiology
  • 1985