A comparison of some kinetic properties of soluble and bound lactate dehydrogenase isoenzymes at different temperatures.

@article{Nitisewojo1976ACO,
  title={A comparison of some kinetic properties of soluble and bound lactate dehydrogenase isoenzymes at different temperatures.},
  author={P Nitisewojo and Herbert O. Hultin},
  journal={European journal of biochemistry},
  year={1976},
  volume={67 1},
  pages={
          87-94
        }
}
A comparison was made of some kinetic properties of three chicken lactate dehydrogenase isoenzymes (1, 3 and 5) at 4, 16, 23 and 40 degrees C. Assays were performed with an enzyme concentration of 0.01 muM at pH 6.0. Under the conditions of assay, lactate dehydrogenase 3 and 5 bound to the particulate fraction of homogenized skeletal muscle and were evaluated in the soluble and particulate state. Binding of isoenzymes 3 and5 to the cellular particulate fraction decreased V. This decrease was… CONTINUE READING