A comparison of sequence resolution on plant chromosomes: PRINS versus FISH

  title={A comparison of sequence resolution on plant chromosomes: PRINS versus FISH},
  author={Matthew Menke and Jorg Fuchs and Ingo Schubert},
  journal={Theoretical and Applied Genetics},
 The resolution of the chromosomal positions of six high- and one low-copy sequences by oligonucleotide-primed in situ (PRINS) labelling was compared with corresponding data obtained after fluorescent in situ hybridization (FISH) on field-bean and barley chromosomes. While PRINS proved to be suitable for the rapid detection of high-copy tandem repeats at the same loci as those revealed by FISH, no clear PRINS signal was obtained for the low-copy family of vicilin genes at their locus on field… CONTINUE READING

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