A comparison of nested polymerase chain reaction and immunofluorescence for the diagnosis of respiratory infections in children with bronchiolitis, and the implications for a cohorting strategy.

@article{Ong2001ACO,
  title={A comparison of nested polymerase chain reaction and immunofluorescence for the diagnosis of respiratory infections in children with bronchiolitis, and the implications for a cohorting strategy.},
  author={G. M. Ong and Dorothy E Wyatt and Hannah. O'Neill and Conall Mccaughey and PeterV. Coyle},
  journal={The Journal of hospital infection},
  year={2001},
  volume={49 2},
  pages={
          122-8
        }
}
Cohorting bronchiolitis patients infected with respiratory syncytial virus (RSV) and/or influenza viruses is paramount in preventing cross-infection of these viruses in hospital. Nested polymerase chain reaction (nPCR) was compared with immunofluorescence (IF) for the detection of RSV subtypes A and B in children with suspected bronchiolitis. Co-infection with influenza A(H3N2), Chlamydia spp. and picornavirus/rhinovirus was also investigated using molecular techniques.A total of 50… CONTINUE READING
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