A comparison of methods for heat-mediated antigen retrieval for immunoelectron microscopy: demonstration of cytokeratin No. 18 in normal and neoplastic hepatocytes.

@article{Xiao1996ACO,
  title={A comparison of methods for heat-mediated antigen retrieval for immunoelectron microscopy: demonstration of cytokeratin No. 18 in normal and neoplastic hepatocytes.},
  author={J C Xiao and Anne Adam and Peter Ruck and Edwin Kaiserling},
  journal={Biotechnic & histochemistry : official publication of the Biological Stain Commission},
  year={1996},
  volume={71 6},
  pages={278-85}
}
Postembedding antigen retrieval is a well established technique for immunoelectron microscopy; however, many antigens cannot be detected without additional unmasking procedures. This study was undertaken to determine whether microwave oven heating, autoclaving, and pressurized boiling, which are well recognized methods of antigen retrieval for light microscopy, and simple boiling can also be used in electron microscopy. We investigated neoplastic and normal hepatocytes using a commercially… CONTINUE READING