A comparison of massively parallel nucleotide sequencing with oligonucleotide microarrays for global transcription profiling

@inproceedings{Bradford2009ACO,
  title={A comparison of massively parallel nucleotide sequencing with oligonucleotide microarrays for global transcription profiling},
  author={James R Bradford and Yvonne Hey and Tim Yates and Yaoyong Li and Stuart D. Pepper and Crispin J. Miller},
  booktitle={BMC Genomics},
  year={2009}
}
RNA-Seq exploits the rapid generation of gigabases of sequence data by Massively Parallel Nucleotide Sequencing, allowing for the mapping and digital quantification of whole transcriptomes. Whilst previous comparisons between RNA-Seq and microarrays have been performed at the level of gene expression, in this study we adopt a more fine-grained approach. Using RNA samples from a normal human breast epithelial cell line (MCF-10a) and a breast cancer cell line (MCF-7), we present a comprehensive… CONTINUE READING
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RNA - Seqmeasurement of expression through massively parallel RNAsequencing

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  • Methods
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