A comparison of gene transfer methods in human dendritic cells.

@article{Arthur1997ACO,
  title={A comparison of gene transfer methods in human dendritic cells.},
  author={Jill F Arthur and Lisa H Butterfield and Michael D Roth and Lynne A. Bui and Sylvia M. Kiertscher and Roy Lau and Steven Dubinett and John A. Glaspy and William John Hannan McBride and James Steven Economou},
  journal={Cancer gene therapy},
  year={1997},
  volume={4 1},
  pages={
          17-25
        }
}
Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs) for the initiation of antigen-specific T-cell activation. DCs may be highly enriched from peripheral blood-adherent leukocytes by short-term (7-day) culture in the presence of interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor. Various methods of gene transfer were studied, including DNA/liposome complexes, electroporation, CaPO4 precipitation, and recombinant adenovirus (AdV) vectors. Low levels of… CONTINUE READING

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