A comparison of critical osmolality and hydraulic conductivity and its activation energy in fowl and bull spermatozoa.

@article{Watson1992ACO,
  title={A comparison of critical osmolality and hydraulic conductivity and its activation energy in fowl and bull spermatozoa.},
  author={Paul F. Watson and Elaine Kunze and P G Cramer and Roy H. Hammerstedt},
  journal={Journal of andrology},
  year={1992},
  volume={13 2},
  pages={
          131-8
        }
}
Measurements were made of critical osmolality, the osmolality at which 50% of the cells are lysed, and of the permeation time, the time taken to lyse 50% of the cells in an osmotic solution lower than the critical osmolality, for fowl and bull spermatozoa. Cell lysis was determined by means of fluorescent viability stains (carboxyfluorescein diacetate and propidium iodide) using a flow cytometer. The advantages and pitfalls of this approach are addressed. The values obtained have been used to… 
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TLDR
Results indicate a temperature-dependent critical tonicity, with sperm exhibiting an increased membrane fragility at 8, 0, and -7 degrees C, relative to 30, 22, -1, -3, or -5 degrees C (67.5 +/- 2.4, [mean +/- SEM], 62.9 +/- 3.3, and 61.8 +/- 5.2 mOsm, respectively).
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