A comparison of changes in nucleotide-protein interactions in the striatal, hippocampus and paramedian cortex after cerebral ischemia and reperfusion: correlations to regional vulnerability.

  title={A comparison of changes in nucleotide-protein interactions in the striatal, hippocampus and paramedian cortex after cerebral ischemia and reperfusion: correlations to regional vulnerability.},
  author={B. Sankaran and J. Clemens and B. Haley},
  journal={Brain research. Molecular brain research},
  volume={47 1-2},
[32P]Azido-purine analogs of ATP and GTP were used to detect changes in phosphorylation and nucleotide binding induced by ischemia and subsequent reperfusion in rat brain striatum, hippocampus and paramedian cortex (PM cortex) tissues. Major changes in phosphorylation were observed for a 130-kDa protein, tentatively identified as the Ca2+ transport ATPase, and calcium/calmodulin-dependent protein kinase II (CaM Kinase II) in all tissues. However, recovery of the phosphorylation of the 130-kDa… Expand
Abnormal properties of creatine kinase in Alzheimer's disease brain: correlation of reduced enzyme activity and active site photolabeling with aberrant cytosol-membrane partitioning.
The report shows that Alzheimer's disease (AD) brain creatine kinase (CK) is modified such that the nucleotide binding site of CK is blocked and that abnormal partitioning of CK between the solubleExpand
Changes in Brain Neurofilament and Beta-Tubulin Proteins after Cerebral Hypoxia-Ischemia in Rabbits
The response of theNF proteins to brain injury is more complicated than described previously and additional studies examining the regulation and metabolism of the NF are warranted, especially regarding the role of phosphorylation. Expand
Glutamine Synthetase and Glutamine Synthetase‐Like Protein from Human Brain
Results from activitymeasurements suggest that the hydroxylamine‐L‐glutamine transferase activity measured routinely in protein extracts from brain is the sum of GS and GSLPactivities, which indicates tighter association with the particulate fraction for GSLP than for GS. Expand
Activity-based probes for functional proteomics.
  • M. Patricelli
  • Biology, Medicine
  • Briefings in functional genomics & proteomics
  • 2002
The general properties and design features of ABPs will be discussed with an emphasis on the use ofABPs for activity-based proteome analysis. Expand


Posthoc phosphorylation of proteins derived from ischemic rat hippocampus, striatum and neocortex
It is concluded that abnormalities of protein phosphorylation may be necessary for ischemic injury to neurons but none are sufficient to explain the selective vulnerability of certain brain regions to isChemic damage. Expand
Brain glutamine synthetase increases following cerebral ischemia in the rat
The results suggest that astrocyte metabolism of glutamate increases after ischemia, and the increased capacity for glutamine synthetase may be important in normalizing extracellular glutamate following ischemIA and protecting brain from the neurotoxic effects of this excitatory amino acid. Expand
Role of calcium in inactivation of calcium/calmodulin dependent protein kinase II after cerebral ischemia
The results suggest that ischemia and reperfusion injury results in the generation of activated oxygen and the mobilization of calcium which inactivate CaM kinase II and indicates that changes associated with protein kinase activity in the brain following an ischemic insult may have profound effects upon neurodegeneration and neuronal survival. Expand
Ca 2+ calmodulin-dependent protein kinase II immunoreactivity in the
It is suggested that CaM kinase II molecules in the CA1 subfield are preferentially located on the CA2+/calmodulin-dependent Kinase II cells in the rat hippocampus and that this plays a critical role in the reconstruction of neuronal cytoskeleton and neuronal networks damaged by ischemic insult. Expand
Effects of ischemia on multifunctional calcium/calmodulin-dependent protein kinase type II in the gerbil.
Cerebral ischemia produces a disruption of calcium homeostasis in neurons. This may explain the extreme sensitivity of these cells to ischemic insult. Prolonged increases in calcium levels mayExpand
Cerebral ischemia decreases endogenous calcium-dependent protein phosphorylation in gerbil brain
The results suggest that an early event in the development of ischemia-induced neuronal death is a decrease in Ca2+-dependent protein phosphorylation. Expand
Efflux of Glutamate Produced by Short Ischemia of Varied Severity in Rat Striatum
It is proposed that the extracellular glutamate concentration may not reach critical levels during short episodes of penumbral ischemia, but this might happen with a longer ischemic period. Expand
Ischemic brain injury selectively alters ATP binding of calcium and calmodulin-dependent protein kinase II.
The observation that ischemia induced an alteration of ATP binding without affecting calmodulin binding is consistent with the hypothesis that is chemia directly affects the ATP binding of CaM kinase II which results in subsequent inhibition of the enzyme. Expand
Oxidative damage to brain proteins, loss of glutamine synthetase activity, and production of free radicals during ischemia/reperfusion-induced injury to gerbil brain.
It is reported that free radical flux is increased during the reperfusion phase of the ischemia-lesioned gerbil brain, and the free radical spin trap N-tert-butyl-alpha-phenylnitrone-dependent nitroxide radical obtained in the lipid fraction. Expand
Detection of glutamine synthetase in the cerebrospinal fluid of Alzheimer diseased patients: a potential diagnostic biochemical marker.
  • D. Gunnersen, B. Haley
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1992
The presence of GS in the CSF of terminal AD patients suggests that this enzyme may be a useful diagnostic marker and that further study is warranted to determine any possible role for glutamate metabolism in the pathology of AD. Expand