A comparison of binding surfaces for SPR biosensing using an antibody-antigen system and affinity distribution analysis.

@article{Zhao2013ACO,
  title={A comparison of binding surfaces for SPR biosensing using an antibody-antigen system and affinity distribution analysis.},
  author={Huaying Zhao and Inna I. Gorshkova and Gregory L. Fu and Peter Schuck},
  journal={Methods},
  year={2013},
  volume={59 3},
  pages={
          328-35
        }
}
The application of optical biosensors in the study of macromolecular interactions requires immobilization of one binding partner to the surface. It is often highly desirable that the immobilization is uniform and does not affect the thermodynamic and kinetic binding parameters to soluble ligands. To achieve this goal, a variety of sensor surfaces, coupling strategies and surface chemistries are available. Previously, we have introduced a technique for determining the distribution of affinities… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 44 references

Biophys

Z. Salamon, G. Lindblom, G. Tollin
J. 84 • 2003
View 16 Excerpts
Highly Influenced

Anal

X. Fan, I. M. White, +3 authors Y. Sun
Chim. Acta 620 • 2008
View 4 Excerpts
Highly Influenced

Surface Chemistry in SPR Technology

View 4 Excerpts
Highly Influenced

Colloid Surf

J. Piehler, A. Brecht, K. Hehl, G. Gauglitz
B 13 • 1999
View 4 Excerpts
Highly Influenced

Curr

P. Schuck, L. F. Boyd, P. S. Andersen
Protoc. Cell Biol. 17 • 1999
View 4 Excerpts
Highly Influenced

M

L. C. Gjelstrup, J. D. Kaspersen
a Behrens, J.S. Pedersen, S. Thiel, P. Kingshott, C.L.P. Oliveira, N.M. Thielens, T. Vorup-Jensen, J. Immunol. 188 (2012) 1292– 1306. H. Zhao et al. / Methods 59 • 2013

Chem

J. N. Cash, E. B. Angerman, +6 authors J. Biol
287 • 2012
View 1 Excerpt

Methods Mol

T. Vorup-jensen
Biol. 757 • 2012