Self-guided slide/tape (ST) and web page (WP) instruction in normal radiographic anatomy of periapical and panoramic images is compared using objective test performance and subjective preference of freshman dental students. A class of seventy-four students was divided into a group studying anatomy in periapical images using WP and a group studying similar ST material. In a modified cross-over design the groups switched presentation technologies to learn anatomy in panoramic images. Students completed self-administered on-line quizzes covering WP materials and conventional quizzes for ST material. Students also completed a voluntary survey. Mean quiz performance identifying matched anatomic features in PA (n = 26) and panoramic images (n = 35) was excellent (96.9%) and not different between image types (p = 0.12) or presentation technologies (p = 0.81). Students preferred WP for accessibility, ease of use, freedom of navigation, and image quality (p < .01). Student comfort level with the quiz formats of the two technologies was not different (p = 0.11). Students experienced a higher rate of mechanical and logistical problems with ST than with WP technology. While 71 percent of the students preferred WP technology, this preference appears to be related to ease of use and facilitation of flexible learning styles rather than improved didactic performance.