A comparison of DNA extraction and purification methods to detect Escherichia coli O157:H7 in cattle manure.

@article{Trochimchuk2003ACO,
  title={A comparison of DNA extraction and purification methods to detect Escherichia coli O157:H7 in cattle manure.},
  author={Teegan Trochimchuk and John Fotheringham and Edward Topp and Heidi Schraft and Kam Tin Leung},
  journal={Journal of microbiological methods},
  year={2003},
  volume={54 2},
  pages={165-75}
}
The extraction of DNA from manure and the subsequent polymerase chain reaction (PCR) amplification of virulence genes to detect pathogens require an effective method of purification. Four different methods were assessed for their effectiveness in extracting and purifying Escherichia coli O157:H7 DNA from cattle manure: phenol/chloroform purification, phenol… CONTINUE READING