A comparative study for ultrastructural localization of intracellular immunoglobulins using peroxidase conjugates.

@article{Kuhlmann1974ACS,
  title={A comparative study for ultrastructural localization of intracellular immunoglobulins using peroxidase conjugates.},
  author={Wolf Dieter Kuhlmann and Stratis Avrameas and Therese Ternynck},
  journal={Journal of immunological methods},
  year={1974},
  volume={5 1},
  pages={33-48}
}
Fixation with 4% formaldehyde/24 hours, 1% formaldehyde and 1% glutaraldehyde/1-1.5 hours and 1-1.5% glutaraldehyde/1-1.5 hours provided satisfactory ultrastructural conservation of the tissues. None of these fixatives appeared to denature IgG globulins, but formation of cross-linkages prevents conjugates from reaching antigenic sites. Weak aldehyde fixation may enhance penetration of conjugates; however, ultrastructural detail is poorly preserved and proteins will leak out from insufficiently… CONTINUE READING

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Weak aldehyde fixation may enhance penetration of conjugates ; however , ultrastructural detail is poorly preserved and proteins will leak out from insufficiently fixed tissue , thus increasing the possibility of staining artifacts .
Weak aldehyde fixation may enhance penetration of conjugates ; however , ultrastructural detail is poorly preserved and proteins will leak out from insufficiently fixed tissue , thus increasing the possibility of staining artifacts .
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