A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli

@inproceedings{Balzer2013ACA,
  title={A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli},
  author={Simone Balzer and Veronika Kucharova and Judith A. Megerle and Rahmi Lale and Trygve Brautaset and Svein Valla},
  booktitle={Microbial cell factories},
  year={2013}
}
BACKGROUND Production of recombinant proteins in bacteria for academic and commercial purposes is a well established field; however the outcomes of process developments for specific proteins are still often unpredictable. One reason is the limited understanding of the performance of expression cassettes relative to each other due to different genetic contexts. Here we report the results of a systematic study aiming at exclusively comparing commonly used regulator/promoter systems by… CONTINUE READING
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