A common precursor for hematopoietic and endothelial cells.

Abstract

Embryonic stem cell-derived embryoid bodies contain a unique precursor population which, in response to vascular endothelial growth factor, gives rise to blast colonies in semi-solid medium. Upon transfer to liquid culture with appropriate cytokines, these blast colonies generate both hematopoietic and adherent, stromal-type cells. Cells within the adherent population display characteristics of endothelial lineage including the expression of CD31, flk-1, flt-1, tie-2, the capacity to take up acetylated LDL and the presence of cytoplasmic Weibel-Palade bodies. Mixing studies demonstrated that the hematopoietic and endothelial precursors within the blast colonies develop from the same cell, the blast colony-forming cell. Kinetic analysis showed that the blast colony-forming cell represents a transient cell population that develops early and is lost quickly during embryoid body development. These findings provide strong evidence that the blast colony-forming cell represents the long-hypothesized hemangioblast, the common precursor of the hematopoietic and endothelial lineages.

6 Figures and Tables

0200400600'99'01'03'05'07'09'11'13'15'17
Citations per Year

6,539 Citations

Semantic Scholar estimates that this publication has 6,539 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Choi1998ACP, title={A common precursor for hematopoietic and endothelial cells.}, author={Kyu Lee Choi and Mark Thomas Kennedy and Alexander R. Kazarov and John C. Papadimitriou and G H Keller}, journal={Development}, year={1998}, volume={125 4}, pages={725-32} }