A common oocyst surface antigen of Cryptosporidium recognized by monoclonal antibodies

@article{Yu2002ACO,
  title={A common oocyst surface antigen of Cryptosporidium recognized by monoclonal antibodies},
  author={J-R. Yu and Stephen O'Hara and J. Lin and Murray Dailey and G. C. Cain},
  journal={Parasitology Research},
  year={2002},
  volume={88},
  pages={412-420}
}
Two hybridoma clones, CMYL3 and CMYL30, were generated by immunizing Balb/c mice with excysted oocysts of Cryptosporidium muris. Both clones secreted monoclonal antibodies against an oocyst-wall antigen with apparent molecular mass of 250 kDa (called CM250) from C. muris and C. parvum. The epitope appeared to be periodate-sensitive, suggesting the involvement of a carbohydrate moiety. Immunofluorescence and confocal microscopy on purified oocysts and infected mouse tissues revealed staining… CONTINUE READING

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