A commercially available multiplex real-time PCR for detection of pathogens in cardiac valves from patients with infective endocarditis.

@article{Leli2014ACA,
  title={A commercially available multiplex real-time PCR for detection of pathogens in cardiac valves from patients with infective endocarditis.},
  author={Christian Leli and Amedeo Moretti and Maria Bruna Pasticci and Elio Cenci and Francesco Bistoni and Antonella Mencacci},
  journal={Diagnostic microbiology and infectious disease},
  year={2014},
  volume={79 1},
  pages={98-101}
}
Infective endocarditis (IE) is a life-threatening condition, burdened by high mortality. Current guidelines recommend that, in case of negative culture result, tissues from excised heart valves or vegetations from patients with suspected IE should be referred for broad-range bacterial PCR and sequencing. In this proof-of-concept study, the diagnostic utility of the commercially available multiplex real-time PCR system SeptiFast (SF), performed on cardiac valves, was evaluated in a selected… CONTINUE READING