A combined vascular-catecholamine fluorescence method reveals the relative vascularity of rat locus coeruleus and the paraventricular and supraoptic nuclei of the hypothalamus.


Vascular perfusion with a fluorescent dye, Pontamine sky blue, was combined with glyoxylic acid-induced catecholamine fluorescence to produce simultaneous staining of intracerebral blood vessels and catecholamine-containing cells and fibers. Quantitative measurements of blood vessels per unit area revealed that the vascular density of rat locus coeruleus… (More)


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