A combined in vitro/bioinformatic investigation of redox regulatory mechanisms governing cell cycle progression.

@article{Conour2004ACI,
  title={A combined in vitro/bioinformatic investigation of redox regulatory mechanisms governing cell cycle progression.},
  author={John E Conour and W. Vallen Graham and H. Rex Gaskins},
  journal={Physiological genomics},
  year={2004},
  volume={18 2},
  pages={196-205}
}
The intracellular reduction-oxidation (redox) environment influences cell cycle progression; however, underlying mechanisms are poorly understood. To examine potential mechanisms, the intracellular redox environment was characterized per cell cycle phase in Chinese hamster ovary fibroblasts via flow cytometry by measuring reduced glutathione (GSH), reactive oxygen species (ROS), and DNA content with monochlorobimane, 2',7'-dichlorohydrofluorescein diacetate (H2DCFDA), and DRAQ5, respectively… CONTINUE READING